Text Tools · Biology
DNA reverse complement, protein and GC%.
Paste a DNA sequence to get its reverse complement, its protein translation in three forward frames, and its GC content. Nothing you enter leaves this page.
Sequence
Result
How it works
- Reverse complement: each base is swapped for its Watson-Crick partner (A with T, C with G), then the order is reversed, so the result reads 5′ to 3′ on the opposite strand.
- Translation: codons are read with the NCBI standard genetic code, table 1 (NCBI Taxonomy, The Genetic Codes). Frames 1, 2 and 3 start at the first, second and third base of the strand you entered.
*marks a stop codon (TAA, TAG, TGA); a trailing partial codon is ignored and a codon containing N shows as X. Other genetic codes (for example vertebrate mitochondrial) are not applied. - GC content: (G + C) divided by total bases, as a percentage. N counts toward length but not toward GC.
- Input: letters A, C, G, T, U and N only. U is read as T. Lines starting with > (FASTA headers) are skipped, and spaces and digits are removed.
- Reverse strand proteins: to translate the opposite strand, copy the reverse complement back into the box.